6FHW

Structure of Hormoconis resinae Glucoamylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-19
Detector	ADSC QUANTUM 210
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	137.975, 149.827, 192.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.580 - 3.600
R-factor	0.2647
Rwork	0.264
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1glm
RMSD bond length	0.007
RMSD bond angle	1.223
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.580	3.760
High resolution limit [Å]	3.600	3.600
Rmeas	0.142	0.675
Number of reflections	38033
<I/σ(I)>	10.5
Completeness [%]	81.6
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	tacsimate