6FHV
Crystal structure of Penicillium oxalicum Glucoamylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-08-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 189.253, 189.253, 115.379 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.000 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.634 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.310 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 53301 | |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PCB Bis-Tris-propane buffer PEG1500; third Silver bullet condition G2 thiodiglycolic acid, adipic acid, benzoic acid,oxalic acid anhydrous, terephtalic acid, 20 mM Hepes |
