6FHI

Crystal structure of bat influenza A/H17N10 polymerase with viral RNA promoter bound to a 19-mer serine 5 phosphorylated Pol II CTD peptide with a truncated linker.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97242
Spacegroup name	C 1 2 1
Unit cell lengths	268.740, 147.520, 88.540
Unit cell angles	90.00, 96.63, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.23205
Rwork	0.230
R-free	0.26978
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m3h
RMSD bond length	0.007
RMSD bond angle	1.013
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Number of reflections	81064
<I/σ(I)>	6.35	1.43
Completeness [%]	96.1	96.4
Redundancy	2.19	2.06
CC(1/2)	0.986	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	Bat FluA polymerase bound to the ends of the RNA promotor (5 prime end nts 1-16, 3 prime end nts 1-18) was co-crystallized in a 1:8 ratio with the serine 5 phosphorylated 19-mer Pol II CTD peptide mimic, with a truncated linker, in 0.8 M sodium-potassium phosphate at pH 5.0. Crystals were set at 4 C using seeding.