6FH8
E. coli surface display of streptavidin for directed evolution of an allylic deallocase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 57.722, 57.722, 183.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.800 - 1.640 |
| R-factor | 0.12208 |
| Rwork | 0.120 |
| R-free | 0.15905 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.312 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.800 | 1.660 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 19391 | |
| <I/σ(I)> | 26.9 | |
| Completeness [%] | 98.3 | |
| Redundancy | 15.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 2.0 M ammonium sulfate, 0.1 M sodium acetate, pH 4.0 |
