6FH4

CtsR C-terminal domain with bound phospho-arginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-02-01
Detector	MARRESEARCH
Wavelength(s)	1.542
Spacegroup name	P 21 21 2
Unit cell lengths	44.100, 135.550, 59.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.899 - 2.490
R-factor	0.2475
Rwork	0.244
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h0d
RMSD bond length	0.007
RMSD bond angle	1.192
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13rc1_2961: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.899	2.579
High resolution limit [Å]	2.490	2.490
Number of reflections	13014
<I/σ(I)>	5.73
Completeness [%]	99.8
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	292.15	10% v/v polypropylene glycol P 400