6FGZ

Cyanidioschyzon merolae Dnm1 (CmDnm1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	80
Detector technology	CCD
Collection date	2006-06-01
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9330
Spacegroup name	P 62 2 2
Unit cell lengths	188.219, 188.219, 163.627
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.838 - 7.002
R-factor	0.3507
Rwork	0.344
R-free	0.41120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bej
RMSD bond length	0.022
RMSD bond angle	2.135
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_1932)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.838	7.380
High resolution limit [Å]	7.000	7.000
Rmerge	0.140
Number of reflections	2963
<I/σ(I)>	19.5
Completeness [%]	99.5
Redundancy	18.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.0 M ammonium phosphate, 0.1 M lithium sulphate and 0.1 M sodium acetate pH 4.6