6FGE

Crystal structure of human ZUFSP/ZUP1 in complex with ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 65 2 2
Unit cell lengths	84.484, 84.484, 201.867
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.160 - 1.740
R-factor	0.17538
Rwork	0.174
R-free	0.20944
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.810
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.930	1.780
High resolution limit [Å]	1.740	1.740
Rmerge	0.082	0.990
Rmeas	0.085	1.020
Number of reflections	44699	2395
<I/σ(I)>	25.8	4
Completeness [%]	100.0	100
Redundancy	29	30.6
CC(1/2)	1.000	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	4% v/v Tacsimate pH 5.0 and 12% w/v Polyethylene glycol 3,350.