6FGD
Crystal structure of Gephyrin E domain in complex with Artemether
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 87.152, 99.455, 111.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.580 - 1.500 |
| R-factor | 0.15 |
| Rwork | 0.149 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5err |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.077 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.580 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.081 | 1.284 |
| Rmeas | 0.096 | 1.527 |
| Rpim | 0.051 | 0.816 |
| Number of reflections | 77361 | |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M sodium acetate 20-36% MPD |
