6FGC

Crystal structure of Gephyrin E domain in complex with Artesunate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-20
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	I 2 2 2
Unit cell lengths	87.100, 99.220, 113.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.550 - 1.500
R-factor	0.144
Rwork	0.143
R-free	0.16200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5err
RMSD bond length	0.009
RMSD bond angle	1.186
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.550	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.048	1.464
Number of reflections	78437
<I/σ(I)>	23.2
Completeness [%]	99.9
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1 M sodium acetate 20-36 % MPD