6FF5
X-ray structure of bovine heart cytochrome c at high ionic strength
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.966 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 83.350, 83.350, 92.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.900 - 1.740 |
| R-factor | 0.16289 |
| Rwork | 0.161 |
| R-free | 0.20255 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b4z |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.075 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.900 | 1.780 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.040 | 0.800 |
| Number of reflections | 12830 | 3810 |
| <I/σ(I)> | 20.4 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6 | |
| CC(1/2) | 0.999 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 3.2-3.4 M ammonium sulfate and 0.4-0.5 M NaNO3 |
