6FF2
CopZ metallochaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 26.619, 49.460, 102.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.410 - 1.300 |
| R-factor | 0.16655 |
| Rwork | 0.165 |
| R-free | 0.20194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qif |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.745 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.410 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 34140 | 1649 |
| <I/σ(I)> | 10.5 | 1.6 |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 4.6 | 4.8 |
| CC(1/2) | 0.999 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM MgCl2, 100 mM HEPES pH 7.0, 20%w/v PEG 6000 |
