6FES
Crystal structure of novel repeat protein BRIC2 fused to DARPin D12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.990, 63.000, 133.540 |
| Unit cell angles | 97.99, 91.92, 110.81 |
Refinement procedure
| Resolution | 44.895 - 3.000 |
| R-factor | 0.2529 |
| Rwork | 0.251 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | one DARPin chain from structure 4ydw |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.659 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.177 | 1.308 |
| Number of reflections | 31567 | |
| <I/σ(I)> | 7.6 | 2.15 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 0.2 M Ammonium sulfate, 0.1M Bis-Tris pH 5.5, 25 % (w/v) PEG 3350 |
