6FDM
Human Rio2 kinase structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-09-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.920, 98.327, 117.069 |
Unit cell angles | 90.00, 95.22, 90.00 |
Refinement procedure
Resolution | 66.640 - 2.100 |
R-factor | 0.2034 |
Rwork | 0.202 |
R-free | 0.23740 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.640 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.022 | 0.336 |
Number of reflections | 331294 | 31840 |
<I/σ(I)> | 20.59 | 2.32 |
Completeness [%] | 98.5 | 96.68 |
Redundancy | 3.8 | 3.7 |
CC(1/2) | 0.999 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | Morpheus screen D2 |