6FDH
Structure of H. salinarum RosR (vng0258) grown from KCl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-16 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9219 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.069, 76.966, 42.129 |
Unit cell angles | 90.00, 110.21, 90.00 |
Refinement procedure
Resolution | 38.540 - 1.850 |
R-factor | 0.1811 |
Rwork | 0.179 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SAD derived model in NaCl |
RMSD bond length | 0.006 |
RMSD bond angle | 0.777 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13rc2_2986) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.500 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.078 | 0.800 |
Rmeas | 0.086 | 0.850 |
Rpim | 0.036 | 0.827 |
Number of reflections | 21011 | 1277 |
<I/σ(I)> | 17.9 | 1.4 |
Completeness [%] | 99.8 | 99 |
Redundancy | 5.7 | 5.67 |
CC(1/2) | 0.990 | 0.522 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | KCL, AMMONIUM SULFATE, MES PH 6.8 |