6FD4
Crystal Structure of Human APRT-Tyr105Phe variant in complex with Adenine, PRPP and Mg2+, 14 hours post crystallization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.380, 47.540, 47.670 |
| Unit cell angles | 69.86, 77.20, 61.76 |
Refinement procedure
| Resolution | 44.640 - 1.500 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5HND |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.348 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.640 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.083 | 0.611 |
| Number of reflections | 51022 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 92.7 | 91.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | NAOAC, PEG4000, GLYCEROL, TRIS, PH 8.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
