6FCW
Catalytic subunit HisG from Psychrobacter arcticus ATP phosphoribosyltransferase (HisZG ATPPRT) in complex with PRATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-10 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5148 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 70.700, 33.940, 91.437 |
| Unit cell angles | 90.00, 104.76, 90.00 |
Refinement procedure
| Resolution | 23.820 - 2.000 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Chain E of 5M8H |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.372 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.820 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.172 | 0.621 |
| Rmeas | 0.193 | 0.704 |
| Rpim | 0.087 | 0.325 |
| Number of reflections | 13619 | 915 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 93.9 | 88.1 |
| Redundancy | 4.7 | 4.3 |
| CC(1/2) | 0.989 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 10mg/ml protein in 20mM Tris HCl pH8.0, 50mM KCl, 10mM MgCl2, 2mM DTT mixed 1:1 with 32% PEG 3350, 0.1M MOPS pH6.5, 0.1M K/Na tartrate |
