6FCM

Crystal structure of human PCNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-29
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.96600
Spacegroup name	P 21 21 21
Unit cell lengths	39.180, 140.060, 169.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.800
R-factor	0.19917
Rwork	0.195
R-free	0.27588
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1axc
RMSD bond length	0.008
RMSD bond angle	1.310
Data reduction software	XDS (VERSION May 1, 2016 BUILT=20160617)
Data scaling software	XSCALE (VERSION May 1, 2016 BUILT=20160617)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.048	0.802
Rmeas	0.056	0.962
Number of reflections	23289	1567
<I/σ(I)>	18.27
Completeness [%]	97.0	92.2
Redundancy	3.43	3.03
CC(1/2)	0.999	0.719

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	19 to 23% PEG 3350, 0.2 to 0.3 M NaCl, 0.1 M Tris-HCl pH 8.5