6FCD
Human Methionine Adenosyltransferase II mutant (R264A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 67.141, 94.230, 116.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.410 - 1.700 |
R-factor | 0.16116 |
Rwork | 0.160 |
R-free | 0.18423 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a1i |
RMSD bond length | 0.015 |
RMSD bond angle | 1.695 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.410 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.124 | 0.513 |
Rmeas | 0.158 | 0.665 |
Rpim | 0.096 | 0.419 |
Number of reflections | 40576 | 2138 |
<I/σ(I)> | 5.9 | 4.1 |
Completeness [%] | 99.0 | 99.8 |
Redundancy | 4.3 | 4.1 |
CC(1/2) | 0.985 | 0.619 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% PEG600 0.1M HEPES pH 7.0 |