6FBT
The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa in complex with the reaction product NAG-anhNAMpentapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 63 |
| Unit cell lengths | 165.526, 165.526, 54.733 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 143.350 - 2.500 |
| R-factor | 0.18298 |
| Rwork | 0.180 |
| R-free | 0.23944 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ohu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.673 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 143.350 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.024 | 0.360 |
| Number of reflections | 30002 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.4M sodium potassium phosphate pH 6.5 |
