6FBQ
Crystal Structure of the Human Retinoid X Receptor DNA-Binding Domain Bound to the Human MEp DR1 Response Element, pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.2821 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.333, 44.257, 62.137 |
| Unit cell angles | 90.00, 98.31, 90.00 |
Refinement procedure
| Resolution | 42.440 - 1.600 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cn5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | PHASER (2.6.0) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.440 | 42.440 | 1.570 |
| High resolution limit [Å] | 1.540 | 8.450 | 1.540 |
| Rmerge | 0.097 | 0.063 | 1.075 |
| Rmeas | 0.123 | 0.078 | 1.391 |
| Rpim | 0.075 | 0.045 | 0.875 |
| Total number of observations | 83577 | ||
| Number of reflections | 35888 | 248 | 1306 |
| <I/σ(I)> | 5.5 | ||
| Completeness [%] | 87.8 | 90.1 | 65.3 |
| Redundancy | 2.3 | 2.1 | 2.1 |
| CC(1/2) | 0.992 | 0.992 | 0.144 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 20% PEG 3350, 0.1M MOPS pH 7.0, 0.1M NH4Cl, 0.1M MgCl2 |
