6FB7

Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with its target DNA in the presence of Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.6
Spacegroup name	P 21 21 2
Unit cell lengths	71.504, 169.153, 49.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.015 - 2.689
R-factor	0.1677
Rwork	0.163
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g9z
RMSD bond length	0.009
RMSD bond angle	0.992
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.020	2.830
High resolution limit [Å]	2.689	2.690
Rmerge	0.100
Number of reflections	30910
<I/σ(I)>	15.1
Completeness [%]	97.1
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol