6FB3
Teneurin 2 Partial Extracellular Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.560, 452.560, 146.360 |
| Unit cell angles | 90.00, 95.12, 90.00 |
Refinement procedure
| Resolution | 89.380 - 2.380 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.24200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.510 | 2.440 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmeas | 0.250 | |
| Number of reflections | 439054 | 29248 |
| <I/σ(I)> | 4.69 | 0.39 |
| Completeness [%] | 96.3 | 86.8 |
| Redundancy | 5.6 | 1.8 |
| CC(1/2) | 0.990 | 0.315 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 291.15 | 0.1 M Tris (base)/Bicine (pH 8.5), 20% v/v glycerol, 10% w/v PEG4K, 30 mM di-ethyleneglycol, 30 mM tri-ethyleneglycol, 30 mM tetraethyleneglycol, and 30 mM pentaethyleneglycol |
