6FAW
Crystal structure of C-terminal modified Tau peptide-hybrid 4.2c-I with 14-3-3sigma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977930 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.849, 70.230, 128.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.842 - 1.400 |
| R-factor | 0.1444 |
| Rwork | 0.143 |
| R-free | 0.17130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hf3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.037 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.18) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.830 | 46.830 | 1.420 |
| High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
| Rmerge | 0.073 | 0.040 | 1.183 |
| Rmeas | 0.077 | 0.042 | 1.235 |
| Rpim | 0.022 | 0.012 | 0.349 |
| Total number of observations | 1388967 | 10035 | 66835 |
| Number of reflections | 111989 | 799 | 5475 |
| <I/σ(I)> | 18.3 | 67.1 | 2.3 |
| Completeness [%] | 99.9 | 99.6 | 99.9 |
| Redundancy | 12.4 | 12.6 | 12.2 |
| CC(1/2) | 0.999 | 0.998 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 25% PEG400, 10 mM HEPES pH 7.5, 5% glycerol, 0.19 M CaCl 2 |
