6FAP

Crystal structure of human BAZ2A PHD zinc finger in complex with Fr23

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9686
Spacegroup name	P 43 21 2
Unit cell lengths	72.796, 72.796, 99.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.730 - 2.700
R-factor	0.21078
Rwork	0.209
R-free	0.24798
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qf2
RMSD bond length	0.005
RMSD bond angle	0.746
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.730	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.148	0.680
Number of reflections	7841	995
<I/σ(I)>	10.7	3.7
Completeness [%]	100.0	100
Redundancy	12	12.3
CC(1/2)	0.990	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	2.4 M sodium/ potassium phosphate