6F9S
Crystal structure of the C-terminal RecA domain of DDX6 in complex with a conserved peptide from LSM14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-09 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979340 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 92.148, 92.148, 149.896 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.070 - 3.030 |
| R-factor | 0.2061 |
| Rwork | 0.204 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2way |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.484 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.070 | 3.140 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmerge | 0.108 | 1.118 |
| Number of reflections | 7824 | 763 |
| <I/σ(I)> | 31.5 | 3.5 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 18.9 | 19.7 |
| CC(1/2) | 0.999 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 1.55 M Li2SO4, 0.1 M Hepes pH 7.5 |
