6F9I
Crystal structure of KLC2 bound to the second tryptophan-acidic motif peptide from calsyntenin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.442, 75.442, 303.356 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.458 - 3.993 |
| R-factor | 0.254 |
| Rwork | 0.253 |
| R-free | 0.27340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zfw |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.976 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.460 | 4.210 |
| High resolution limit [Å] | 3.990 | 3.990 |
| Number of reflections | 8641 | |
| <I/σ(I)> | 6.28 | |
| Completeness [%] | 94.9 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.893 M sodium potassium tartrate, 0.2 M NaCl, 0.1 M imidazole pH 8.0 |
