6F8P

Crystal structure of Gn from Rift Valley fever virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	37.390, 94.180, 97.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.990 - 1.600
R-factor	0.1667
Rwork	0.165
R-free	0.19890
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.525
Data reduction software	xia2
Data scaling software	xia2
Phasing software	autoSHARP
Refinement software	PHENIX (dev_2283)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.995	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.086	1.044
Number of reflections	46607	3369
<I/σ(I)>	12.35
Completeness [%]	99.9	99.6
Redundancy	9.7	9.47
CC(1/2)	0.998	0.818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	20% w/v PEG 6000, 0.1 M HEPES pH 7.0
1	VAPOR DIFFUSION, SITTING DROP	7	298	20% w/v PEG 6000, 0.1 M HEPES pH 7.0