6F8D
Crystal structure of Human ARS2 residues 171-270 + 408-763 (P65 form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.984 |
Spacegroup name | P 65 |
Unit cell lengths | 105.210, 105.210, 267.040 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 91.110 - 3.480 |
R-factor | 0.26537 |
Rwork | 0.264 |
R-free | 0.29224 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f7j |
RMSD bond length | 0.007 |
RMSD bond angle | 0.962 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.110 | 3.570 |
High resolution limit [Å] | 3.480 | 3.480 |
Number of reflections | 20970 | |
<I/σ(I)> | 8.71 | 0.8 |
Completeness [%] | 97.9 | 86.6 |
Redundancy | 3.12 | 2.98 |
CC(1/2) | 0.997 | 0.253 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 277 | Crystals of ARS2 were obtained at 4 C in 2 microlitre hanging drops with a 1:1 ratio of protein solution (6 mg/ml in 20 mM HEPES, 300 mM NaCl, 2 mM tris(2-carboxyethyl)phosphine pH 7.8) to crystallisation solution. The crystallisation solution was 1.2 M sodium dihydrogen phosphate, 0.8 M dipotassium hydrogen phosphate, 0.2 M lithium sulphate, 0.1 M CAPS pH 10.5 |