6F7P
Crystal structure of Human ARS2 residues 147-270 + 408-763
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-31 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.966 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 136.640, 136.640, 158.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 118.330 - 3.700 |
R-factor | 0.27606 |
Rwork | 0.274 |
R-free | 0.30909 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f7j |
RMSD bond length | 0.007 |
RMSD bond angle | 1.014 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 118.330 | 3.800 |
High resolution limit [Å] | 3.700 | 3.700 |
Number of reflections | 18658 | |
<I/σ(I)> | 12.11 | 1.44 |
Completeness [%] | 99.7 | 98.2 |
Redundancy | 6.33 | 6.16 |
CC(1/2) | 1.000 | 0.763 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 281 | Crystals were obtained at 4 C in 2 microlitre hanging drops with a 1:1 ratio of protein solution at 6 mg per ml in 20 mM HEPES, 300 mM NaCl, 2 mM tris(2-carboxyethyl)phosphine pH 7.8) to crystallisation solution. The crystallisation solution was 0.2 M lithium sulphate and 20% (w/v) PEG 3550. |