6F7K
Crystal structure of Dettilon tailspike protein (gp208)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 153.422, 160.827, 161.717 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.760 - 2.100 |
R-factor | 0.18312 |
Rwork | 0.183 |
R-free | 0.22068 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f7d |
RMSD bond length | 0.012 |
RMSD bond angle | 1.225 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.760 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.017 | 0.229 |
Number of reflections | 231572 | 36799 |
<I/σ(I)> | 10.6 | 0.78 |
Completeness [%] | 99.5 | 98.7 |
Redundancy | 6.72 | |
CC(1/2) | 0.998 | 0.452 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.85M Succinic acid pH 7.0, 0.1 M HEPES pH 7.0, 1 %(w/v) PEG 2000 MME |