6F7D
Crystal structure of Dettilon tailspike protein (gp208)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.91841, 1.009, 1.072 |
Spacegroup name | I 21 3 |
Unit cell lengths | 151.623, 151.623, 151.623 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.523 - 1.634 |
R-factor | 0.16 |
Rwork | 0.159 |
R-free | 0.18770 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.126 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1-2575_2579: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.950 | 1.730 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmeas | 0.010 | 0.083 |
Number of reflections | 138026 | 21954 |
<I/σ(I)> | 9.57 | 1.59 |
Completeness [%] | 99.0 | 97.5 |
Redundancy | 3.51 | |
CC(1/2) | 0.997 | 0.639 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1M succinic acid pH 7.0, 0.1 M HEPES pH 7.0, 1 %(w/v) PEG 2000 MME |