6F75

Carboxypeptidase T mutant L254N with Sulphamoil Leucine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.8
Spacegroup name	P 63 2 2
Unit cell lengths	158.930, 158.930, 105.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.120 - 1.890
R-factor	0.15744
Rwork	0.156
R-free	0.18114
RMSD bond length	0.009
RMSD bond angle	1.385
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.120	1.939
High resolution limit [Å]	1.890	1.890
Number of reflections	59832
<I/σ(I)>	4.2538
Completeness [%]	100.0	100
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		296	1,4 SA