6F74
Crystal structure of VAO-type flavoprotein MtVAO713 from Myceliophthora thermophila C1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2015-11-03 |
Detector | MARRESEARCH |
Wavelength(s) | 1.574 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.195, 108.506, 135.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.250 - 2.200 |
R-factor | 0.19793 |
Rwork | 0.196 |
R-free | 0.23859 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MtVAO615 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.575 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.300 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.148 | 0.483 |
Rpim | 0.114 | 0.479 |
Number of reflections | 120921 | 5895 |
<I/σ(I)> | 3.9 | 1.6 |
Completeness [%] | 98.8 | 97.3 |
Redundancy | 2.1 | 2.1 |
CC(1/2) | 0.968 | 0.532 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 25% PEG3350, 0.1 M Tris pH 8.5-9.0 |