6F5P
A mechanism for the activation of the influenza virus transcriptase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 185.700, 185.700, 597.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 131.310 - 4.140 |
| R-factor | 0.2618 |
| Rwork | 0.260 |
| R-free | 0.29580 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.812 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 132.400 | 4.210 |
| High resolution limit [Å] | 4.100 | 4.140 |
| Rpim | 0.055 | 0.568 |
| Number of reflections | 75524 | |
| <I/σ(I)> | 7.9 | 0.9 |
| Completeness [%] | 100.0 | 60.6 |
| Redundancy | 13.5 | 2.1 |
| CC(1/2) | 0.998 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 70% (v/v) Morpheus G2 (Molecular Dimensions) and 7.5% (v/v) glycerol |
