6F4R

Human JMJD5 (N308C) in complex with Mn(II), NOG and RCCD1 (139-143) (complex-3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.429, 64.810, 77.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.840 - 1.300
R-factor	0.1436
Rwork	0.143
R-free	0.16160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f4p
RMSD bond length	0.016
RMSD bond angle	1.401
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.840	1.350
High resolution limit [Å]	1.300	1.300
Rmerge	0.079	1.200
Rpim	0.031	0.553
Number of reflections	62115	6098
<I/σ(I)>	24.3	1.7
Completeness [%]	100.0	100
Redundancy	9	8.8
CC(1/2)	0.998	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	298	0.1M Bis-Tris pH 5.8, 25 % PEG3350, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 1:1 ratio)