6F3T

Crystal structure of the human TAF5-TAF6-TAF9 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0044
Spacegroup name	I 2 3
Unit cell lengths	339.050, 339.050, 339.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.915 - 2.500
R-factor	0.1984
Rwork	0.197
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.658
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.915	100.000	2.600
High resolution limit [Å]	2.500	50.000	2.500
Rmerge	0.136	0.051	3.048
Rmeas	0.153	0.064	3.491
Number of reflections	219180	21	22436
<I/σ(I)>	8.67	16.93	0.64
Completeness [%]	99.0	70	91.7
Redundancy	4.69	2.857	4.146
CC(1/2)	0.995	0.992	0.188

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277.15	0.1 M Tris/HCl pH6.8, 0.8M Na-Citrate, 0.0-0.15M NaCl