6F3F
Autoinhibited Src kinase bound to ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-11 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.011, 82.974, 105.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.526 - 2.418 |
| R-factor | 0.210911359312 |
| Rwork | 0.208 |
| R-free | 0.26764 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2src |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.583 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.510 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Number of reflections | 17674 | |
| <I/σ(I)> | 6.1 | |
| Completeness [%] | 99.5 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 50 mM PIPES, pH 6.5 1.2 M sodium tartrate 20 mM DTT 2 mM ADP 5 mM MgCl2 |
