6F39
C1r homodimer CUB1-EGF-CUB2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.563, 54.620, 138.180 |
| Unit cell angles | 90.00, 99.90, 90.00 |
Refinement procedure
| Resolution | 68.061 - 5.801 |
| R-factor | 0.3096 |
| Rwork | 0.308 |
| R-free | 0.33790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.357 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2722) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.100 | 6.000 |
| High resolution limit [Å] | 5.800 | 5.800 |
| Rpim | 0.118 | |
| Number of reflections | 2987 | |
| <I/σ(I)> | 5.1 | 1.8 |
| Completeness [%] | 99.8 | |
| Redundancy | 15.2 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 12-18% PEG 8000, 100 mM Imidazole at pH 8.0 |
