6F1R
Tetragonal Lysozyme crystallized at 298 K and pH 4.5 with phosphate bound: control experiment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.694, 78.694, 37.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.438 - 1.100 |
| R-factor | 0.1542 |
| Rwork | 0.154 |
| R-free | 0.16350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e3u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.928 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 18.830 | 18.830 | 1.120 |
| High resolution limit [Å] | 1.100 | 6.020 | 1.100 |
| Rmerge | 0.047 | 0.040 | 0.603 |
| Rmeas | 0.050 | 0.043 | 0.644 |
| Rpim | 0.018 | 0.017 | 0.223 |
| Total number of observations | 396478 | ||
| Number of reflections | 48271 | 267 | 2378 |
| <I/σ(I)> | 20.2 | ||
| Completeness [%] | 99.6 | 72.8 | 100 |
| Redundancy | 8.2 | 6.5 | 8 |
| CC(1/2) | 0.998 | 0.995 | 0.907 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.5 M Sodium Chloride, 0.1 M Sodium Acetate |
