6F1M

Lysozyme crystallized in presence of 100 mM sodium phosphate at pH 4.5: low-humidity form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	30.104, 55.522, 66.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.497 - 1.480
R-factor	0.2275
Rwork	0.226
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e3u
RMSD bond length	0.008
RMSD bond angle	0.992
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.500	19.500	1.500
High resolution limit [Å]	1.480	8.090	1.480
Rmerge	0.092	0.055	0.527
Rmeas	0.102	0.061	0.595
Rpim	0.043	0.026	0.266
Number of reflections	17334	117	933
<I/σ(I)>	9
Completeness [%]	90.9	83	98.3
Redundancy	4.8	4.8	4.6
CC(1/2)	0.994	0.993	0.664

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	0.5 M Sodium Chloride, 0.1 M Sodium Phosphate. 0.1 M Sodium Acetate