6F1G
The structure of AbnB-E201A, an intracellular 1,5-alpha-L-arabinanase from Geobacillus stearothermophilus, in complex with arabinopentaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.960, 89.210, 40.839 |
Unit cell angles | 90.00, 102.45, 90.00 |
Refinement procedure
Resolution | 29.730 - 1.672 |
R-factor | 0.1622 |
Rwork | 0.160 |
R-free | 0.19460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d5y |
RMSD bond length | 0.006 |
RMSD bond angle | 1.009 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.700 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.079 | 0.530 |
Number of reflections | 34562 | 1699 |
<I/σ(I)> | 28.8 | 1.9 |
Completeness [%] | 99.9 | 98.6 |
Redundancy | 3.8 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.9-2.1 M LiSO4, 0.1 M Tris pH 8.5, 3% PEG400, 5mM arabinopentose |