6F13
GLIC mutant E75A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 190 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.948, 132.450, 159.234 |
| Unit cell angles | 90.00, 101.95, 90.00 |
Refinement procedure
| Resolution | 49.100 - 2.700 |
| R-factor | 0.2037 |
| Rwork | 0.203 |
| R-free | 0.22270 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 100065 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 100 mM NaAcetate pH 4 200mM NaSCN 12-15% PEG4000 15% glycerol 3%DMSO |
