6F03
The crystal structure of secreted antigen BPSL2520
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9790 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.955, 52.731, 136.122 |
Unit cell angles | 90.00, 93.84, 90.00 |
Refinement procedure
Resolution | 38.954 - 2.200 |
R-factor | 0.2045 |
Rwork | 0.202 |
R-free | 0.24450 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.861 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX ((1.10.1_2155-0000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.122 | 0.636 |
Number of reflections | 36157 | 35764 |
<I/σ(I)> | 8.9 | 3.1 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.7 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30 % PEG 4000, 0.2 M ammonium acetate, 0.1M HEPES pH 7.0. 20% glycerol added for cryoprotection |