6EZR

Crystal structure of GH20 Exo beta-N-Acetylglucosaminidase from Vibrio harveyi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9998
Spacegroup name	P 1 21 1
Unit cell lengths	90.150, 130.710, 98.480
Unit cell angles	90.00, 112.99, 90.00

Refinement procedure

Resolution	90.660 - 2.370
R-factor	0.2166
Rwork	0.214
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rcn
RMSD bond length	0.007
RMSD bond angle	1.272
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.660	90.660	2.430
High resolution limit [Å]	2.370	10.600	2.370
Rmerge	0.146	0.058	1.087
Rmeas	0.159	0.063	1.173
Total number of observations	577490
Number of reflections	84783	985	6189
<I/σ(I)>	9.93	21.25	2.21
Completeness [%]	99.4	97.8	99.1
Redundancy	6.811	6.621	7.051
CC(1/2)	0.994	0.997	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.5	293	0.1 M sodium acetate pH 4.6, 1.4 M sodium malonate