6EYV
Crystal structure of the pyoverdine maturation protein PvdP in complex with the mock substrates L-tyrosine and zinc.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-13 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.332, 109.143, 82.510 |
| Unit cell angles | 90.00, 95.66, 90.00 |
Refinement procedure
| Resolution | 48.092 - 2.704 |
| R-factor | 0.2096 |
| Rwork | 0.205 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eys |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.460 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2875)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.092 | 3.032 |
| High resolution limit [Å] | 2.701 | 2.701 |
| Rmeas | 0.320 | |
| Rpim | 0.101 | 0.536 |
| Number of reflections | 23084 | 1154 |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 92.2 | 56.5 |
| Redundancy | 9.9 | 7.5 |
| CC(1/2) | 0.988 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.857 M NH4SO4 0.1 M MES pH 5.5 0.5 M ZnCl2 1 mM L-tyrosine |
