6EYP

X-ray structure of the unliganded uridine phosphorylase from Vibrio cholerae at 1.22A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.886
Spacegroup name	P 32
Unit cell lengths	92.940, 92.940, 140.231
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.480 - 1.220
R-factor	0.138
Rwork	0.138
R-free	0.17000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oeh
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.480	1.300
High resolution limit [Å]	1.220	1.220
Rmerge	0.124	1.065
Rmeas	0.131	1.125
Total number of observations	4015025
Number of reflections	399225	63823
<I/σ(I)>	10.75	1.88
Completeness [%]	99.7	98.6
Redundancy	10.05	9.59
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0,2 M MgCl2,6H2O, 15%(w/v) PEG 4000, 0,1 M TRIS -HCl