6EYE
Crystal structure of murine neuroglobin under 150 bar krypton
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.8556 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.854, 87.854, 113.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.410 - 1.700 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.25394 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4o4t |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.241 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.410 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.043 | 0.275 |
| Rmeas | 0.045 | 0.318 |
| Rpim | 0.013 | 0.151 |
| Number of reflections | 18730 | 2672 |
| <I/σ(I)> | 28.3 | |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 19.6 | 4.7 |
| CC(1/2) | 0.999 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.6M AMMONIUM SULPHATE, 0.1M MES |
