6EXZ

Crystal structure of Mex67 C-term

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 61
Unit cell lengths	71.419, 71.419, 20.343
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.925 - 1.300
R-factor	0.1714
Rwork	0.170
R-free	0.20670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oai
RMSD bond length	0.009
RMSD bond angle	1.203
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.930	1.350
High resolution limit [Å]	1.300	1.300
Rmerge	0.045
Rmeas	0.046
Number of reflections	14960
<I/σ(I)>	28.54
Completeness [%]	100.0	100
Redundancy	19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	35% Tacsimate pH 7