6EXC
Crystal structure of DotM cytoplasmic domain (residues 153-380),R314E/R315E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.430, 50.630, 55.440 |
| Unit cell angles | 102.52, 97.67, 95.50 |
Refinement procedure
| Resolution | 53.440 - 2.160 |
| R-factor | 0.1995 |
| Rwork | 0.196 |
| R-free | 0.26013 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6exb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.440 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 25233 | |
| <I/σ(I)> | 5.7 | 1.3 |
| Completeness [%] | 97.1 | 95.3 |
| Redundancy | 1.8 | 1.7 |
| CC(1/2) | 0.494 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M potassium chloride 0.1 M HEPES pH 7.5 15% w/v PEG 6000 |
