6EX0
Crystal structure of RelP (SAS2) from Staphylococcus aureus bound to pppGpp in the post-catalytic state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 124.330, 124.330, 213.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.700 - 2.780 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ded |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2614: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 2.879 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.073 | 1.048 |
| Rmeas | 0.080 | 1.168 |
| Rpim | 0.030 | 0.492 |
| Number of reflections | 133228 | 10390 |
| <I/σ(I)> | 12.63 | 1.4 |
| Completeness [%] | 99.3 | 93.11 |
| Redundancy | 6.3 | 5.1 |
| CC(1/2) | 0.997 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Hepes-Na pH7.0 0.2M Sodium thiocyanate 40% pentaerythriol propoxylate (5/4 PO/OH) |
